| abstract |
Stimulable dissolvable PEG hydrogel comprising a matrix of PEG polymers, which are modified to contain at least one multifunctional fusion protein, multifunctional fusion protein comprising as components a substrate binding peptide (SBP), a binding peptide Repetitive GDPR, preferably a marker for purification and at least one N- and / or C-terminal connector; where the at least one multifunctional fusion protein is covalently bound to the PEG polymer, where the substrate binding peptide (SBP) is selected from the N-terminal sequence of the B subunit of the Escherichia coli gyrase DNA (GyrB), of the N-terminal sequence of subunit B of the gyrase DNA of Escherichia coli according to SEQ ID No. 2 or an amino acid sequence showing at least 80% identity with the sequence according to SEQ ID No. 2, or between FKBP, FM, ToxT, DHFR, Cyp, E, PIP, TetR, ArgR, ArsR, HucR, FRB, streptavidin, avidin, neutravidin, steroid hormone receptor, steroid hormone or heparin binding protein proteins; where the N- and / or C-terminal connector is selected from a thiol-containing moiety, a thiol-modified amino acid or thiol-containing, a cysteine, a homocysteine, a maleimide-coupled thiol, a vinyl sulfone moiety, peptide sequences, peptide bonds, a HaloTag, a SNAP-tag, a CLIP-tag, a reaction bond of transglutaminase, amino acids or NTA chelate formation entities or a multivalent metal ion binding polyhistidine, and where the gel formation is performed by joining the Substrate binding peptide (SBP) with specific substrate. |