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publicationDate 2013-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber ES-2410754-T3
titleOfInvention Procedure for electrochemical detection of binding reactions
abstract Procedure for the realization of homogeneous competitive immunoassay formats with electrochemical detection in solution, characterized in that two different conjugates are combined as reagents with the sample or a mixture of sample buffer, a conjugate comprising a redox marker and an analyte molecule and comprising the second conjugate a redox anti-marker antibody or a fragment thereof that specifically binds and a molecule that specifically binds to the analyte, and because the presence of free analyte in the sample allows or promotes the binding of the redox marker to the anti-marker antibody redox, inhibiting ("extinguishing") through this union the redox activity of the attached redox marker, so that a detectable signal is generated or modified.
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