http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2400302-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9f935b84827ff4a3e441c3f219d841de |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1037 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2001-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2da2ef311b950a0ee1354f8747e57f5f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6fddec0fe1fd64beb8d1736b26750526 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_09a5817d94b70252678644bc0d7246f0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_81b45d4e3cfef3b2da753a7a9a73557a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c18a188e41881925dd5b2d8a26629ec0 |
| publicationDate | 2013-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2400302-T3 |
| titleOfInvention | New methods for building gene package libraries that collectively present members of a diverse family of peptides, polypeptides or proteins |
| abstract | A method for preparing nucleic acids, the method comprising the steps of: (i) amplifying a nucleic acid using a complementary primer of at least part of a synthetic sequence located at the 5 'end of the nucleic acid sequence; (ii) make the single stranded amplified nucleic acid; (iii) contacting the amplified single-stranded nucleic acid with a single-stranded oligonucleotide, the single-stranded oligonucleotide being complementary to the single-stranded nucleic acid in a region in which cleavage is desired, in which the single-stranded nucleic acid and the single-stranded oligonucleotide are associated to form a region Locally double-stranded single-stranded nucleic acid, in which the locally double-stranded region comprises a restriction endonuclease recognition site, and (iv) cleave the nucleic acid at the restriction endonuclease recognition site, in which the cleavage comprises contacting a restriction endonuclease with the locally double stranded region, in which the restriction endonuclease is specific to the restriction endonuclease recognition site, and in which the cleavage removes all unwanted 5 'nucleotides from the nucleic acid; the contact and cleavage stages being performed at a temperature between 45 ° C and 75 ° C, in which the nucleicomonocatenary acid and the single stranded oligonucleotide are associated to form a locally double stranded region of the single stranded nucleic acid and in which the restriction endonuclease is active at selected temperature between 45 ºC and 75 ºC. |
| priorityDate | 2000-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 82.