| abstract |
An aptamer possessing inhibitory activity against midkine, which is either (a) or (b) below: (a) an aptamer comprising a nucleotide sequence selected from SEQ ID NO: 1 to 2, 4 to 20,22 at 33, 35 to 36, 39 to 64 and 66 to 70, with the proviso that uracil can be thymine, where the nucleotides contained in the aptamer are such that, (i) the 2 'positions of the pyrimidine nucleotides, and they are identical or different, they are fluorine atoms or are substituted by atoms or groups selected from the group consisting of hydrogen atoms, hydroxy groups and methoxy groups, and (ii) the 2 'positions of the purine nucleotides, whether identical or different, they are hydroxy groups or are substituted by atoms or groups selected from the group consisting of hydrogen atoms, methoxy groups and fluorine atoms; (b) an aptamer comprising a nucleotide sequence selected from SEQ ID NO: 1 to 2, 4 to 20.22 to 33, 35 to 36, 39 to 64 and 66 to 70, with the proviso that the uracil can be thymine, wherein one to three nucleotides are substituted, deleted, inserted or added, wherein the nucleotides contained in the aptamer are such that, (i) the 2 'positions of the pyrimidine nucleotides, whether identical or different, are fluorine atoms or are substituted by atoms or groups selected from the group consisting of hydrogen atoms, hydroxy groups and methoxy groups, and (ii) the 2 'positions of purine nucleotides, whether identical or different, are hydroxy groups or are substituted by atoms or groups selected from the group consisting of hydrogen atoms, methoxy groups and fluorine atoms. |