http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2396387-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_955b6ab72e27fe0c106d21e1f4e7b13b http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_26db05f349a12e10706b1e5ea02c860a |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-44778 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-26 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 |
filingDate | 2011-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4a6e06bf49462b5d64e2b087ad3fed7e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ddaeb48d5c4af47f1928068d302a6824 |
publicationDate | 2013-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | ES-2396387-A1 |
titleOfInvention | PROCEDURE FOR IDENTIFICATION OF CARBON STONES. |
abstract | The present invention is a method of identifying carbonylated histones, which comprises electrophoretically separating said histones in a gel comprising acrylamide, Triton X-100, glacial acetic acid and urea, incubating said gel with 2,4-dinitrophenyl hydrazine (DNPH), electrophoretically separating said histones in a gel comprising acrylamide, Tris and sodium dodecyl sulfate, transferring said histones from the previous gel to an adsorbent membrane, detecting the presence of 2,4-dinitrophenyl (DNP) in said adsorbent membrane and identifying said carbonylated histones and its variants The invention also provides a carbonylated histone identification kit comprising reagents for preparing a TAU gel, reagents for preparing an SDS-PAGE gel, 2,4-dinitrophenyl hydrazine, specific primary antibody against DNP and secondary antibody labeled against the primary antibody . |
priorityDate | 2011-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 84.