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publicationDate 2012-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber ES-2393667-T3
titleOfInvention Inhibitors of alpha-amylase activity
abstract An α-amylase activity inhibitor comprising, as an effective component, polyphenols obtained by the following procedure: guava leaves and / or guava fruit are extracted with one or more solvents selected from water and hydrophilic solvents ; The resulting extract is subjected to ultrafiltration to thereby eliminate substances having a molecular weight of less than 5,000; the remaining fraction is applied to the hydrophobic chromatography column with butyl support and the elution is carried out by using an aqueous solution of sodium dihydrogen phosphate (0.02 mol / l) and an aqueous solution of sodium phosphate ( 0.02 mol / l) (flow rate: 1 ml / minute) in a pH gradient between the two solutions and a fraction of the eluted substance is recovered, a fraction that corresponds to the third individual peak of an elution curve obtained when The absorbance of the substance is measured at 260 nm
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