http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2391931-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b8ae50a1ac4cd76423a4efc15db872d1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dc365d0d4cf537a503859144cd924ad6 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6854 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate | 2009-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2012-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c394993f1eb0561a126542e1a7dd7402 |
| publicationDate | 2012-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2391931-T3 |
| titleOfInvention | Procedures for quantifying protein filtration from protein-based affinity chromatography resins |
| abstract | A method for quantifying protein filtration from a protein-based affinity chromatography resin, said protein being selected from Protein A (PrA), Protein G (PrG) and Protein L (PrL), the method comprising the steps of: (a) label the protein-based chromatography resin with one or more fluorescent labels; (b) divide the labeled resin into a first and second equal parts; (c) treat the first part with a suitable means to release the labeled protein from the resin and add an excess amount of immunoglobulin (Ig) to the second part; (d) measure the fluorescence (FLdigest) and protein concentration ([protein]) of the resin treated in the first part of step (c); and (e) measuring the concentration of Ig eluate of the resin ([Ig]) and the fluorescence signal in an Ig eluate (FLIg) of the second part of step (c), in which the protein filtration it is quantified by calculating {(FLIg) / ((FLdigest) / [protein]))} / [Ig]. |
| priorityDate | 2008-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 94.