http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2391837-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_829870c3d1719dc25f6939097d79a0e3 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6855 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2006-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2012-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c36240f244291a4a5df0cad33a208165 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e5821d1faa25c8575ab0eda62ee2bb37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ffa8bfd1ca6d9b9718e6cc591d12066 |
| publicationDate | 2012-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2391837-T3 |
| titleOfInvention | Method for the detection of high performance AFLP-based polymorphisms |
| abstract | Method for the discovery, detection and high performance genotyping of one or more genetic markers in one or more samples, comprising the steps of: (a) providing DNA from one or more samples; (b) cutting the DNA with at least a restriction endonuclease to produce restriction fragments; (c) link adapters to restriction fragments to produce adapter-bound restriction fragments; (d) amplify adapter-bound restriction fragments with a pair of primers that is complementary to the adapters to produce preamplified adapter bound restriction fragments; (e) amplifying preamplified adapter bound restriction fragments with a pair of primers, containing at least one of the primers from one to 10 selective nucleotides at its 3 'end and containing at least one of the primers an identifier tag at the 5 'end of the primer to produce an amplifiable subset library labeling of restriction fragments linked to adapter for each sample; (f) optionally grouping the libraries; (g) sequencing the libraries, sequencing comprising the steps of: - matching the restriction fragments linked to adapter with beads, each bead being paired with a single adapter-linked fragment; - emulsify the beads in water-in-oil microreactors, each water-in-oil microreactor comprising a single pearl; - perform an emulsion PCR to amplify the adapter-bound restriction fragments on the surface of the beads; - load the pearls into wells, each well comprising a single pearl; and - generate a pyrophosphate signal; (h) group the sequences by library, using the identification tag; (i) identify genetic markers within the library and / or between libraries (j) determine (co) dominant genotypes of the genetic markers in one or more libraries. |
| priorityDate | 2005-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 39.