http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2391078-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9b7e3799d02776160735934b621d0747 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_136cb56216d220f184305e921e1ac221 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-16 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23K1-165 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 |
| filingDate | 2005-10-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2012-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e18a3807db0d9f1cdac9f4f716fe0db2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c2b5915b894386dcd8b020d11521bca4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_455c0d15e56a6e96178afe8d1ec2f98f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb359e546e63353a2a420a73fe988661 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db821ccb155df6451bbbd0cc12b8bdd9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9ee5aa70fdf0ea2879fdaab8bee61454 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8c8c5cf346946885d36922c70d69174 |
| publicationDate | 2012-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2391078-T3 |
| titleOfInvention | Phytase |
| abstract | Method of preparing a variant of the phytase enzyme, said method comprising the following sequential steps: a) selecting at least one mother phytase enzyme, wherein the at least one mother phytase enzyme is a polypeptide selected from the group consisting of: a polypeptide comprising the amino acid sequence presented in SEQ ID No. 3 or an amino acid sequence that has an identity of at least 80% with it; a polypeptide that can be obtained from strain P1-29 of Buttiauxella sp. deposited with access number NCIMB 41248; a polypeptide that can be obtained by the expression of SEQ ID No. 2 or a nucleotide sequence that can be obtained from strain P1-29 of Buttiauxella sp. deposited with the accession number NCIMB 41248 or a nucleotide sequence having a sequence identity of at least 80% with it; and a polypeptide that can be obtained from strain P1-29 of Buttiauxella sp. deposited with the accession number NCIMB 41248 and wherein the peptide is obtained by the expression of SEQ ID No. 2 or a nucleotide sequence that can be obtained from strain P1-29 of Buttiauxella sp. deposited with the accession number NCIMB 41248 or of a nucleotide sequence having a sequence identity of at least 80% with it, wherein said polypeptide has phytase activity, wherein said polypeptide has a specific activity of at least 300 U / mg, wherein said specific activity is determined by incubating said polypeptide in a solution containing 2 mM phytate, 0.8 mM CaCl 2 in 200 mM sodium acetate buffer at pH 3.5, at a temperature from 37 ° C; b) generating at least one phytase variant by introducing at least one alteration of said mother phytase enzyme which is an insertion, a deletion or a substitution or combination thereof, of an amino acid residue in said mother phytase enzyme to obtain the less a variant of the phytase enzyme; c) scrutinize for said at least one variant of the phytase enzyme to identify an improved phytase enzyme variant which, in comparison to the mother phytase enzyme, has at least one or several improved properties selected from: i. greater thermostability; and / or ii. greater specific activity; and / or 30 iii. greater proteolytic stability; d) preparing said improved phytase enzyme variant |
| priorityDate | 2004-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 334.