http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2386514-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_00690ab9d2654bb8aec02a82abcb86c6 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2009-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2012-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d376ab947695b91cc639c17848f72c3c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_22d29bcca7b4f6b8ae1397a677fb1a37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_637fa4fb1a76a71c9a0f7c7912154b7d |
| publicationDate | 2012-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2386514-T3 |
| titleOfInvention | Fast, highly sensitive isothermal method for the detection of point mutations and SNPs |
| abstract | Method for detecting the presence of a point mutation of a target nucleic acid molecule in a background of natural nucleic acid molecules, comprising the steps of: a. obtain a sample of nucleic acid; b. contacting said nucleic acid sample, under appropriate reaction conditions, with a solution comprising a mixture of oligonucleotides and a DNA polymerase with chain displacement activity under hybridization conditions, wherein said oligonucleotide mixture consists of primers suitable for amplification. loop-mediated isothermal region of the nucleic acid molecule region theoretically including point lamutation, said primers comprising: i) two external primers F3 and B3; ii) two internal primers FIP and BIP, in which FIP includes two sequences of F2 oligonucleotides and F1c, and BIP including oligonucleotide sequences, B2 and B1c, in which said internal primers FIP and BIP can recognize to be inhibited to two different and opposite regions, F2c and B2c respectively, of the target nucleic acid molecule, in which the BIP primer is designed to hybridize downstream of the point mutation, or the priming or FIP is designed to hybridize upstream of the point mutation, and in the event that the BIP primer is designed to hybridize downstream of the point mutation, then the FIP primer is designed to hybridize to the target sequence so that the point mutation is located at or downstream of the sequence of F2c and upstream of F1c, or in the event that the FIP primer is designed to hybridize upstream of the point mutation, then the BIP primer is designed to hybridize to the target sequence so that the point mutation is located or upstream of the sequence of B2c and downstream of B1c; iii) a self-aligned extensible primer LB or LF respectively, comprising: - a central loop sequence that can selectively recognize and hybridize to the region comprising the mutation theoretical point of the nucleic acid molecule only if the point mutation is present, - a 5 'end sequence, and - a 3 'end sequence, said 5' end and 3 'end sequences being complementary to each other to form a stem, so that said central loop sequence has a higher hybridization affinity for the region comprising the theoretical point mutation of the nucleic acid molecule that the hybridization affinity of the 5 'end sequence with respect to the 3' end sequence, so that as a result an alignment and amplification of the region comprising the theoretical point mutation of the acid molecule nucleic acid; iv) a non-extensible moiety to selectively recognize and hybridize to the WT (natural) sequence of the nucleic acid molecule; c. incubate the resulting mixture at a constant temperature; d. detecting an indicative signal of amplification of the nucleotide molecule comprising the point mutation. |
| priorityDate | 2009-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 47.