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publicationDate 2012-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber ES-2378352-T3
titleOfInvention Procedure to analyze a blood sample or a sample of a blood derivative using a hematological system based on flow cytometry
abstract A method for analyzing a blood sample or a sample of a blood derivative using a hematological system based on flow cytometry comprising an optical detection system, the method comprising: providing a closed consumable tube (60) having at one end thereof septum that is pierceable by a needle, said tube containing: a known number of reference particles, each of said reference particles having a predetermined diameter and being distinguishable from the target cells of the blood sample or the blood derivative sample based in at least one optical measurement of an extinction measurement, a measurement of anterograde dispersion of the small angle light, a measurement of the right angle dispersion, a measurement of the dispersion of the high angle light and a measurement of the duration Of flight; and at least one reagent to allow the determination of the cellular composition of the blood sample or blood derivative sample, the at least one reagent comprising at least one dye; pierce the septum with a needle (11) and aliquot a first portion of whole blood and a known volume of enveloping solution in the closed consumable tube (60) through the needle (11), forming the first portion of whole blood, the known volume of envelope solution, the reference particles and the at least one reagent, a first solution; suctioning a known volume of the first solution from the closed consumable tube (60) in the optical detection system through the needle (11); use the optical detection system to measure light scattering and light absorption by erythrocytes, platelets, and individual reference particles within the volume of the first solution, and to measure light absorption of at least one dye within the known volume of the first solution; aliquot a second portion of blood and a volume of enveloping solution in the closed consumable tube (60) through the needle (11); aliquot a lysate aliquot into the consumable tube (60) through the needle (11), forming the contents of the closed consumable tube (60) and the lysate, a second solution; suction a known volume of the second solution in the optical detection system through the needle (11): use the optical detection system to measure light scattering and light absorption by leukocytes, particles of reference and the at least one individual dye within the known volume of the second solution.
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