http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2371202-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e0bd2564785dbfbd379992a7ee616c6f |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2007-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2011-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b5aa34a88d40bbfca8d9f0ee61f57c2c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d1bbff2a2a907d870da6312fc406609 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e0f3e33ed006de7fc7e6c1390e647169 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c931cf79a775909ca90cfe7bd012f925 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3fece931d349e1e125027c052aa02d65 |
| publicationDate | 2011-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2371202-T3 |
| titleOfInvention | METHOD OF DIAGNOSIS AND FOLLOW-UP OF A BACTERIAL VAGINOSIS BY MOLECULAR QUANTIFICATION. |
| abstract | Diagnostic method and in vitro monitoring of the status of vaginal bacterial flora against the presence of a bacterial vaginosis and, where appropriate, for the follow-up of its therapeutic treatment, characterized in that - 1 / bacteria concentrations are quantified Atopobium vaginae and Gardnerella vaginalis * determining the concentrations of specific sequences of said bacteria Atopobium vaginae and Gardnerella vaginalis present in a single copy in the DNA of said bacteria Atopobium vaginae and Gardnerella vaginalis respectively, and of a specific sequence of a human gene present in any biological extraction containing human cells, in the DNA extracted from a sample of vaginal secretion of the patient, said specific sequences having a size smaller than 150 nucleotides, * by enzymatic co-amplification of PCR type of said specific sequences contained, on the one hand, in said DNA extracted from the sample and, on the other hand, in samples of synthetic DNA fragments comprising each of said specific sequences said bacteria and said specific sequence of a human gene present in any biological extraction of human cells, said samples of quantification calibration standard serving of the DNA, * the detection and quantification of said fragments being performed with the help of probes labeled with sequences other than those of the amplification primers for each of said specific sequences of said bacteria Atopobium vaginae and Gardnerella vaginalis and said specific sequence of a human gene present in any biological extraction containing human cells, * said specific sequences of said bacteria being the following sequences, which include sequences of said (underlined) probes flanked by sequences of said primers (in bold) or their complementary sequences : - for At opobium vaginae: - for Gardnerella vaginalis: - 2 / the presence of a bacterial vaginosis or the failure of the ongoing therapeutic treatment is determined, with a positive predictive value of at least 95% and a negative predictive value of at least 99% , if the concentrations of DNA fragments of the two specific sequences of the bacteria Atopobium vaginae and respectively Gardnerella vaginalis in a sample of extraction of vaginal secretions from a patient containing at least 10 4 human cells / ml, are such that a at least of the following 2 conditions a) and b): a) the Ca concentration in said Atopobium vaginae DNA fragment is greater than or equal to 10 8 copies / ml, and b) the Cg concentration in said Gardnerella vaginalis DNA fragment is greater than or equal to 10 9 copies / ml. |
| priorityDate | 2006-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 419.