abstract |
A non-therapeutic method of disinfection, comprising the steps of treating a surface, a suspension or a solution contaminated with a PrP Sc prion protein or a substitute thereof simultaneously with a combination of (1) Carlsberg subtilisin, and ( 2) or: a) Dodecylbenzenesulfonic acid (DOBS) Teric 164 and sonication; b) sodium dodecyl sulfate (SDS), Empigen BS / AU and sonication; c) SDS, borax and an increased temperature up to 150 ° C; d) DOBS and Triton X100; or e) dimethyl sulfoxide (DMSO) at a concentration at which the unfolding is irreversible; where DOBS, SDS, DMSO, temperature and sonication favor the conformational deployment of PrP Sc prion protein, while not denaturing Carlsberg subtilisin, and Teric 164, Empigen BS / AU, borax and Triton X 100 promote or protect the folding of Carlsberg subtilisin, without preventing excision of the prion protein. |