abstract |
A method for obtaining a bifunctional complex comprising a presentation molecule and an identifying oligonucleotide comprising labels that identify the reagents that have participated in the formation of the presentation molecule, said method comprising the steps of a) providing a nascent bifunctional complex that comprises a chemical reaction site and a priming site for the enzymatic addition of a label in the form of a sequence of consecutive nucleotides, b) reacting the chemical reaction site with one or more reagents, and c) reacting the priming site enzymatically with one or more labels that identify the one or more reagents, in which a reagent and the label that identifies the reagent are not bound before their reaction with the chemical reaction site and the priming site, respectively, of the bifunctional complex nascent. |