http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2368152-T3
Outgoing Links
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|---|---|
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| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0636 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P17-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P37-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P37-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P37-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P29-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P9-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-0008 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-12 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0783 |
| filingDate | 2005-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2011-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_98e1a14dbda64a47b5b968df73c541b0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e449ce288adb969bc321319889285f7a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_827d3ab1759f30f91bf6e90b65e60af8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9e6a128217d6d6e137dce7ae5d9d3383 |
| publicationDate | 2011-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2368152-T3 |
| titleOfInvention | OBTAINING TR1 CELLS SPECIFIC TO FOOD ANTIGENS OR SELF-ORIGINS FROM A POPULATION OF LEUKOCYTES OR PBMC. |
| abstract | An in vitro method for obtaining a population of specific Tr1 cells of food antigens or autoantigens from a population of leukocytes or a population of peripheral blood mononuclear cells (PBMC), said method comprising: 1) stimulating the population of PBMC or leukocytes with the food antigen or autoantigen, 2) recover the population of specific Tr1 cells from food antigens or autoantigens from the population of stimulated cells, 3) expand the population of specific Tr1 cells from recovered food antigens or autoantigens in step (2) in a culture medium Mp, where said expansion step comprises: a) cultivating at a temperature T1 in a culture medium Mf, feeder cells capable of expressing the factors of group (A) comprising: - a modified anti-CD3 antibody, where the modification of the anti-CD3 antibody consists in the replacement of the intracytoplasmic domain anti-CD3 of the anti-CD3 heavy chain with a transmembrane domain, said modified anti-CD3 antibody being anchored to the cell membrane of the feeder cells and being susceptible to interacting with the CD3 / TCR protein complex of the T cells, - the CD80 or CD86 protein, preferably the CD80 protein, anchored to the cell membrane of the feeder cells, which is susceptible to interact with the CD28 protein of the T cells, and - the CD58 protein anchored to the cell membrane of the feeder cells, which is susceptible to interact with the CD2 protein of Tr1 cells, - IL-2 secreted by feeder cells, which is susceptible to interact with the IL-2 receptor of Tr1 cells, and - an interleukin selected from IL-4 or interleukin 13 (IL-13), preferably IL-4, said interleukin being secreted by feeder cells and being susceptible to interact with the IL-4 receptor of Tr1 cells, so that That T1 allows the proliferation of said feeder cells, b) contacting the feeder cells obtained in step (a) purified or not of their Mf culture medium, with the population of Tr1 specific antigen cells contained in the culture medium Mp, wherein said Mp culture medium does not initially contain said group (A) of factors in order to obtain a mixture containing the population of Tr1 antigen-specific cells, feeder cells and the Mp culture medium, c) cultivate the mixture obtained in step (b) containing the factors of group (A) that are expressed by feeder cells in the culture medium Mp, where said step (c) of cultivation is carried out at a temperature T2 which is by at least about 35 ° C, such that: - the population of antigen-specific Tr1 cells proliferates, and - the feeder cells do not proliferate, and where the population of antigen-specific Tr1 cells is expands, d) recovering the population of Tr1 specific antigen cells thus expanded. |
| priorityDate | 2005-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 2021.