| abstract |
Procedure for the preparation of an active component of a vaccine in the absence of serum, comprising stages in which (a) MDCK cells are infected with a virus, the virus being a flu virus from a primary isolate, which was multiplied previously in cell culture to obtain a homogeneous seeding virus for productive purposes, (b) MDCK cells in suspension culture are grown on an industrial scale under conditions that make it possible to multiply the viruses, the culture being carried out at a volume of at least 30 l. |