| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c4dabde38d5f5072744e8bd4aa2f266b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7076dedc2d2a657bb9fccb6f5dbf0296 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-0231
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-36
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-42
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-06 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 |
| filingDate |
2010-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2012-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b9a55c16450ca0e2013e140d04dee81c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1e67cd735c60f128c1b06f46f0dd6a9a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b5496e8550f1c409524dbf3c06c22e1d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c85d2c787e253257f27fe6113a4c0b6f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_263e0e9cb101faf9f3ffb93de035aca3 |
| publicationDate |
2012-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
ES-2363551-B2 |
| titleOfInvention |
OPTIMIZED HISTOLOGICAL METHOD FOR THE PRESERVATION OF ANTIGENIC EPITHOPES AND THE CELLULAR ARCHITECTURE OF VERTEBRATE FABRICS. |
| abstract |
Optimized histological method for the preservation of antigenic epitopes and the cellular architecture of vertebrate tissues, characterized in that it combines cryosubstitution fixation and inclusion in a wax or paraffin of low melting temperature. The method object of the present invention improves most of the current histological methods designed for optical microscopy. This method also improves the antigenic properties of embryonic and adult tissues of amphioxus, Danio rerio and mouse, preserving their structure and preventing the degradation of RNA. This method is a good alternative to the classical histology methods normally used in embryogenesis studies of vertebrates in the face of such common problems as epitope lability or the intercalation of rigid tissues into soft tissues. |
| priorityDate |
2010-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |