http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2361188-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_966a6c1f382ec9bcb5e3839dfd73e5b8 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-625 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 |
| filingDate | 2006-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2011-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d39e5dd07e2eec8351d0451c0b619fa |
| publicationDate | 2011-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2361188-T3 |
| titleOfInvention | METHOD FOR THE RECOMBINANT EXPRESSION OF A POLYPEPTIDE. |
| abstract | Method for the recombinant production of a heterologous polypeptide in a eukaryotic host cell comprising an expression plasmid, characterized in that: a) the expression plasmid comprises, in the 5 'to 3' direction, aa) a promoter, ab) a nucleic acid encoding a first polypeptide that is a signal sequence, the amino acid sequence from which it is selected from Table 1 depending on the first two amino acids of the second polypeptide selected so that the first two amino acids of the second polypeptide are identical to the first two amino acids of the amino acid sequences of the naturally occurring immunoglobulin FR1 region, ac) a nucleic acid encoding a second polypeptide comprising: i) a nucleic acid encoding said heterologous polypeptide, ii) a nucleic acid encoding a linker, iii) a nucleic acid encoding an immunoglobulin fragment comprising at least in the constant domains of an immunoglobulin chain, ad) a 3 'untranslated region comprising a polyadenialization signal, b) the expression plasmid is introduced into a eukaryotic host cell, c) the host cell is cultured under suitable conditions for the expression of the second polypeptide, d) the second polypeptide is recovered from the culture medium. |
| priorityDate | 2005-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1043.