http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2358296-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efb35e154fff5d3177ae8520d41046d5 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6865 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2007-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2011-05-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_66bd1a46c4c250f12fbfd4529f4dd32a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b0cd595f4d60edfe980d6a75e96d3f9d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_844b9f3451df324312684d61ec2b89a6 |
| publicationDate | 2011-05-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2358296-T3 |
| titleOfInvention | MARKED OLIGONUCLEOTIDES AND USE OF THE SAME IN METHODS OF AMPLIFICATION OF NUCLEIC ACIDS. |
| abstract | A method for the selective amplification of at least one target nucleic acid sequence from a nucleic acid sample, and said method comprises the steps of: (a) treating a nucleic acid sample comprising a target nucleic acid sequence with a labeled oligonucleotide comprising first and second regions, and said first region comprises a target hybridization sequence that hybridizes to a 3 'end of said target nucleic acid sequence, and said second region comprises a 5' marker sequence relative to said target hybridization sequence, wherein said second region does not stably hybridize to a target nucleic acid containing said target nucleic acid sequence; (b) removing and / or inactivating in said nucleic acid sample the unhybridized labeled oligonucleotide having an active form in which a hybridization sequence for the purpose of said unhybridized labeled oligonucleotide is available for hybridization to said nucleic acid sequence objective; (c) following step (b), initiating an extension reaction from the 3 'end of said labeled oligonucleotide hybridized to said target nucleic acid sequence with a DNA polymerase to produce a primer extension product that includes the label sequence and a region complementary to said target nucleic acid sequence; (d) separating said primer extension product from said target nucleic acid; and (e) producing amplification products in a nucleic acid amplification reaction by using first and second oligonucleotides, wherein said first oligonucleotide comprises a hybridization sequence that hybridizes to a 3 'end of the complement of said acid sequence target nucleic, and said second oligonucleotide comprises a hybridization sequence that hybridizes to the complement of said marker sequence, wherein said second oligonucleotide stably hybridizes to said target nucleic acid, and wherein each of said amplification products comprises a base sequence that is substantially identical or complementary to the base sequence of said target nucleic acid sequence, and which further comprises a base sequence that is substantially identical or complementary to all or a portion of said marker sequence. |
| priorityDate | 2006-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 289.