http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2357131-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_15d42f684941d5b0617ca7764ebdff07 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-864 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C02F1-42 |
| filingDate | 2004-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2011-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f61b14737c8a76226442661e2dcff340 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7f0a4000b43394f4bb244d46e18968fb |
| publicationDate | 2011-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2357131-T3 |
| titleOfInvention | PROCEDURES TO PRODUCE PREPARATIONS OF VIRIONS OF AAV RECOMBINANTS SUBSTANTIALLY EXEMPT FROM EMPTY CAPSIDES. |
| abstract | A method for purifying AAV vector particles from an AAV preparation comprising AAV vector particles and empty AAV capsids, to provide a product substantially free of empty AAV capsids, said method comprising: a) providing a host cell comprising AAV vector particles; b) lyse said host cell to obtain a crude cell lysate comprising AAV vector particles and empty AAV capsids; c) applying said crude cell lysate to a first cation exchange chromatography column under conditions in which said AAV vector particles and said empty AAV capsids are attached to the column; d) eluting said AAV vector particles and said AAV empty capsids under non-separation conditions to provide an AAV preparation comprising AAV vector particles and empty AAV capsids; e) applying the AAV preparation from (d) to a second column of cation exchange chromatography under conditions under which said AAV vector particles and said empty AAV capsids are attached to the column; f) adding a low salt buffer to the column of (e) under conditions in which the AAV vector particles are eluted and the empty AAV capsids remain attached to the column; and g) collecting the eluted fractions of (f) comprising AAV vector particles to provide an AAV product substantially free of empty AAV capsids. |
| priorityDate | 2003-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 386.