http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2353137-T3

Outgoing Links

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assignee http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f93b8a5de44029dcd6150a84ded68ca4
classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
filingDate 2007-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2011-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_34a713427a3d86ef62f0f30633610f43
publicationDate 2011-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber ES-2353137-T3
titleOfInvention PROCEDURE FOR PARALLEL INSULATION OF BICATENARY AND MONOCATENARY NUCLEIC ACIDS, AS WELL AS FOR THE SELECTIVE ELIMINATION OF BICATENARY NUCLEIC ACIDS OF A BICATENARY AND MONOCATENARY NUCLEIC ACIDS.
abstract Procedure for the parallel isolation of bi and single stranded nucleic acids, as well as for the selective elimination of double stranded nucleic acids from a mixture of bi and single stranded nucleic acids or from sources containing these substances, characterized by the following steps: a) the sample lysate or homogenized is adjusted in the absence of alcohol or alkaline earth ions complexing substances or detergents, dispersants or humectants with an aqueous saline solution with a concentration greater than 1 M so that the double stranded nucleic acid adsorbs to a solid support, while the Single stranded nucleic acid is not adsorbed and remains in the solution b) Removal of the support with the adsorbed nucleic acid c) The solution with the single stranded nucleic acid is mixed with an alcoholic solution with a concentration of 1 to 90% by volume and placed in contact with another solid support, the monocatena nucleic acid being adsorbed I laugh at this other support.
priorityDate 2006-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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