http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2353137-T3
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f93b8a5de44029dcd6150a84ded68ca4 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate | 2007-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2011-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_34a713427a3d86ef62f0f30633610f43 |
publicationDate | 2011-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | ES-2353137-T3 |
titleOfInvention | PROCEDURE FOR PARALLEL INSULATION OF BICATENARY AND MONOCATENARY NUCLEIC ACIDS, AS WELL AS FOR THE SELECTIVE ELIMINATION OF BICATENARY NUCLEIC ACIDS OF A BICATENARY AND MONOCATENARY NUCLEIC ACIDS. |
abstract | Procedure for the parallel isolation of bi and single stranded nucleic acids, as well as for the selective elimination of double stranded nucleic acids from a mixture of bi and single stranded nucleic acids or from sources containing these substances, characterized by the following steps: a) the sample lysate or homogenized is adjusted in the absence of alcohol or alkaline earth ions complexing substances or detergents, dispersants or humectants with an aqueous saline solution with a concentration greater than 1 M so that the double stranded nucleic acid adsorbs to a solid support, while the Single stranded nucleic acid is not adsorbed and remains in the solution b) Removal of the support with the adsorbed nucleic acid c) The solution with the single stranded nucleic acid is mixed with an alcoholic solution with a concentration of 1 to 90% by volume and placed in contact with another solid support, the monocatena nucleic acid being adsorbed I laugh at this other support. |
priorityDate | 2006-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 52.