http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2352333-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9be85312aba36707fe2b107d38fae77c |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-13 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H6-045 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6895 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H1-00 |
| filingDate | 2004-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2011-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6d6bb67a420fed7a419f7f1d681a0c7d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d214a887ca517abecf8bddeaa3204e98 |
| publicationDate | 2011-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2352333-T3 |
| titleOfInvention | RESISTANCE TO VELLOUS ONION MILLDIU CAUSED BY THE FUNGUS PERONOSPORA. |
| abstract | Plant of the Allium cepa species that is resistant to downy onion powdery mildew caused by the fungus Peronospora destructor (Pd) due to a locus of resistance to Pd present homozygously on the long arm of chromosome 3 in the genome of said plant, in in which the locus of resistance to Pd is the introgression fragment of Allium roylei in the genome of a plant of line 3591-1, registration number NCIMB 41249, and in which the following procedure is carried out: a) restricting the genomic DNA of said plant with the restriction enzymes PstI and MseI; b) perform a ligation with the following oligonucleotide adapters: alpha 5'-CTCGTAGACTGCGTACATGCA CATCTGACGCATGT-5 ', and beta 5'-GACGATGAGTCCTGAG TACTCAGGACTCAT-5'; c) selectively amplify sets of restriction fragments, characterizing the presence of the Pd resistance locus by &8226; a 61 nucleotide amplification product when the following primers are used in step c): (A) 5'-GACTGCGTACATGCAGAAC and 5'-GATGAGTCCTGAGTAACTT, &8226; a 151 nucleotide amplification product when the following primers are used in step c): (B) 5'-GACTGCGTACATGCAGAAG and 5'-GATGAGTCCTGAGTAAAAC and &8226; a 330 nucleotide amplification product when the following primers are used in step c): (C) 5'-GACTGCGTACATGCAGACA and 5'-GATGAGTCCTGAGTAACCA. |
| priorityDate | 2004-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID454482060 http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID198285 |
Total number of triples: 20.