| abstract |
Recombinant protein comprising a cysteine knot domain with an amino acid identity of at least 70% with respect to the 102 aa cysteine knot domain of human GDF-5 as shown in amino acids 400-501 of FIG. 1 or SEQ ID NO 1, wherein a) in said recombinant protein, the amino acid in the position corresponding to arginine 438 (R438) of wild-type human GDF-5 as shown in SEQ ID NO 1 is alanine, valine , leucine, isoleucine or methionine, and / or b) in said recombinant protein, the amino acid of the position corresponding to asparagine 445 (N445) of wild-type human GDF5 as shown in SEQ ID NO 1 is serine or threonine. |