| abstract |
A microorganism as a carrier of nucleotide sequences for antigens and protein toxins comprising the following components: (I) at least one nucleotide sequence encoding at least one complete or partial antigen of at least one wild-type or mutated protein; and (II) at least one nucleotide sequence encoding at least one protein toxin and / or at least one subunit of a protein toxin; and (III) a) at least one nucleotide sequence encoding at least one transport system that allows expression of the expression products of component (I) and component (II) on the outer surface of the microorganism and / or allows the secretion of the expression products of component (I) and component (II); and / or encoding at least one signal sequence that allows the secretion of the expression products of component (I) and component (II); and / or b) optionally, at least one nucleotide sequence encoding at least one protein to lyse the microorganism in the cytosol of mammalian cells and to intracellularly release plasmids or expression vectors, which are contained in the lysed microorganism; and (IV) at least one nucleotide sequence for at least one activation sequence for the expression of one or more of the components (I) to (III), wherein said activation sequence can be activated in the microorganism and / or is tissue cell specific, tumor cell specific, macrophage specific, dendrite specific, lymphocyte specific, function specific or without cell specificity; where any of the components (I) to (IV) may be present once or several times and if a component of the components (I) to (IV) is present several times it may be independently identical or different; and where component (I) and component (II) are not identical, that is component (I) does not encode at least one nucleotide sequence encoding at least one protein toxin and / or at least one subunit of a protein toxin . |