| abstract |
Keratinocytes, characterized in that they are not immortalized, and can be duplicated at least 150 times by an in vitro cell culture procedure, and because they are KC-BI-1 culture cells (DSM ACC 2514), or derived keratinocytes thereof, wherein said keratinocytes cannot be duplicated a. in the absence of fetal calf serum, and / or b. in the absence of Feeder cells and / or c. in the absence of epidermal growth factor (EGF) and where the telomerase activity of the aforementioned keratinocytes compared to the HaCaT cell line is lower, at least by a factor 2, and where the cells and cultures of the derived keratinocytes are generate and / or can be generated by sub-passages and / or subcloned from the original KC-BI-1 crop (DSM ACC 2514). |