| abstract |
A method for preparing live Birnavirus, wherein Birnavirus is infectious bursitis virus (IBDV), which comprises the following steps: - preparing cDNAs of segments A and B of the Birnavirus genome, - transcribing said cDNAs to produce synthetic transcripts RNA, wherein said RNA transcripts are positive-sense RNA transcripts of said segments A and B, - transfect host cells with said synthetic RNA transcripts, incubate said host cells in a culture medium, and - isolate infectious Birnavirus I live from said culture medium. |