http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2341772-T3
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_27a71ddaaa2f5a8c228fc6cb3f3b3098 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-918 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-573 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N- http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-56 |
filingDate | 2004-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2010-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1ea6471b7a998a9940934c56a8140cda http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0924ccff4f9b5b8c66d229b83b096cb2 |
publicationDate | 2010-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | ES-2341772-T3 |
titleOfInvention | HIGH PERFORMANCE TEST TO DETERMINE THE ACTIVITY OF LP-PLA2. |
abstract | A high performance method for determining the activity of a phospholipase enzyme associated with lipoprotein A2 (Lp-PLA2) in a set of samples comprising the steps of; (i) prepare a solution comprising labeled platelet activation factor (PAF); (ii) take aliquots of each of the samples in the set into a well of a microtiter plate or microcentrifuge tube; (iii) contacting each of the tissue samples in the assembly with the solution of the preparation stage; (iv) contacting each of the solutions of the first stage of contact with a PAF sequestrant molecule to form a PAF sequestrant molecular complex; (v) contacting said PAF sequestrant molecular complex with a precipitation solution comprising trichloroacetic acid (TCA) to form a precipitate and a supernatant; (vi) removing said PAF sequestrant molecular complex by centrifuging said microtiter plate or microcentrifuge tube at at least 6,000 g for at least 5 minutes at less than 10 ° C; and (vii) detect Lp-PLA2 activity, where at least one sample has been taken from an animal that has been given an Lp-PLA2 inhibitor. |
priorityDate | 2003-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 75.