| abstract |
Homologous nucleic acid recombination method, the method comprising: (i) aligning homologous sequences of target nucleic acids using a computer program to select the conserved regions of sequence identity and regions of sequence diversity, (ii) synthesize a set of Overlapping oligonucleotides of family gene transposition, where the set of oligonucleotides corresponds to at least one region of sequence diversity in the target nucleic acids, and where the set of oligonucleotides collectively corresponds to 50% or more of the full length of each one of the homologous target nucleic acids, (iii) hybridize the family gene transposition oligonucleotide set, (iv) elongate the family gene transposition oligonucleotide set using a polymerase, thereby providing a population of recombinant nucleic acids, (v ) denature the acid population recombined nucleic acids, thereby providing denatured recombined nucleic acids, (vi) hybridizing again the denatured nucleic acids, (vii) extending the recombined and newly hybridized nucleic acids resulting using a polymerase, (viii) repeating steps (v) to ( vii) at least once, and (iv) select one or more of the recombined and newly hybridized nucleic acids that result for a desired character or property, where non-equimolar proportions of familial transposition oligonucleotides are used to skew recombination. |