| abstract |
A mutant of a CPC acylase composed of an α subunit according to SEQ ID NO: 4 and a β subunit according to SEQ ID NO: 5, or a derivative thereof of functionally equivalent CPC acylase, characterized in that at least one amino acid selected from the group consisting of Val121α, Gly139α and Phe169α of the α subunit of CPC acylase of SEQ ID NO: 4 and Phe58β, His70β, Ile75β, Ile176β and Ser471β of the β subunit of CPC acylase of SEQ ID NO: 5 is replaced by another amino acid, wherein the CPC acylase mutant or the functionally equivalent CPC acylase derivative thereof catalyzes the conversion of a compound of Formula I to a compound of Formula II: ** (See formula) ** where, R it is acetoxy (-OCOCH3), hydroxy (-OH), hydrogen (-H) or a salt thereof. |