http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2337952-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_49310188df8dc04b3708491df863459f http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1e70c0aeade92d7571d955d3b90ea06e |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2561-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2531-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A40-146 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-075 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6813 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8286 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2007-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2010-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0966995fc545305ac123bb3dcc117712 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4e32eec807e75c9ef0907259b1f249ca http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e2d389db95fae76246dead34fe4576d2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_420df2a4ff4be6ed6948ee5d75fc7b5a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3bc68e5c864cf25926c842e2b71cbc30 |
| publicationDate | 2010-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2337952-T3 |
| titleOfInvention | METHOD FOR IDENTIFYING NEW GENES. |
| abstract | A method for identifying pesticidal genes, the method comprising: a) designing at least one pair of oligonucleotide primers for use in a first round of PCR that is specific for a target group of pesticidal genes, the primer pair comprising a direct primer and a reverse primer, where each primer is directed to a distinctive sequence present in the nucleotide sequences of the target group; b) obtain a first sample of nucleic acid material from a microorganism of interest; c) mixing the first sample of nucleic acid material with the at least one pair of oligonucleotide primers for use in the first round of PCR and a thermostable DNA polymerase under conditions that are suitable for PCR amplification; d) perform a first round of PCR and detect the PCR amplification products, thereby determining whether PCR products are produced in the first round of PCR; e) obtain a second sample of nucleic acid material from the microorganism if PCR products are detected in the first round of PCR; f) design at least one pair of oligonucleotide primers for use in a second round of PCR that is specific to the target group of pesticidal genes, the primer pair comprising a direct primer and a reverse primer, where each primer is directed to a distinctive sequence present in the nucleotide sequences of the target group; g) mixing the second sample of nucleic acid material with the at least one pair of oligonucleotide primers for use in the second round of PCR and a thermostable DNA polymerase under conditions that are suitable for PCR amplification and performing a second round of PCR; h) separating any PCR amplification product produced in the second round of PCR using agarose gel electrophoresis and isolating the nucleic acid fragments for further analysis, where the nucleic acid fragments may comprise an alleged novel pesticidal gene fragment; i) cloning each nucleic acid fragment into a cloning vector; j) transforming the host cells with the cloning vectors, where the cloning vectors comprise the nucleic acid fragments isolated in step (h); k) preparing nucleic acid samples from individual host colonies comprising a cloning vector; l) subjecting the nucleic acid samples of the individual host colonies to point-to-transfer analysis using labeled probes that are specific to all known pesticidal genes of the target group, where a nucleic acid fragment isolated in step (h) that is not detected during the stage of punctual transfer analysis comprises a supposed novel pesticidal gene fragment; and m) analyze the supposed novel pesticidal gene fragment. |
| priorityDate | 2006-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 359.