abstract |
Method for the production of a non-transgenic plant, which is resistant or tolerant to a herbicide of the phosphonomethylglycine family comprising: (a) the introduction into plant cells of a recombinant oligonucleobase with an objective mutation in the EPSPS gene to produce plant cells with a mutant EPSPS gene that expresses an EPSP protein that is mutated in one or more amino acid positions, said positions being selected in the group consisting of Leu173, Ala179, Met180, Arg181, Ser98, Ser255 and Leu198 in Arabidopsis EPSPS protein or in an amino acid residue analogous in an EPSP gene of another species; (b) the identification of a plant cell that exhibits normal growth, compared to a corresponding wild-type plant cell in the presence of glyphosate; and (c) the regeneration of a non-transgenic plant resistant or tolerant to the herbicide possessing said mutated EPSPS gene from said plant cell; where the non-transgenic, herbicide-resistant or tolerant plant exhibits normal growth and development compared to that of the corresponding wild-type plants and where the mutated EPSPS enzyme has the same catalytic activity compared to that of the wild-type enzyme. |