http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2335947-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8d6f4380f8fc2bdfe87664beea0bc3d2 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8209 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 1999-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2010-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_050b51ee0a0cd20b2774cafa897c42e8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_68b0ccbad07a384ec54447ff13ba77ed |
| publicationDate | 2010-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2335947-T3 |
| titleOfInvention | HIGH EFFICIENCY TRANSFORMATION OF COTTON MEDIATED BY AGROBACTERIUN USING PECIOL EXPLANTS. |
| abstract | A process for producing a transgenic cotton plant comprising the steps of: (a) obtaining cotton petiole explants, (b) exposing the petiole explants to an Agrobacterium tumefaciens culture that houses a vector comprising an exogenous gene and a selectable marker gene, the Agrobacterium being able to effect the stable transfer of the exogenous gene and the selectable marker gene to the genome of the petiole explant cells, (c) culturing the petiole explants in a medium containing 2,4-dichlorophenoxyacetic acid at a concentration that varies from more than 0 to 0.5 mg / l and quinetin to a concentration that varies from more than 0 to 1 mg / l to induce callus formation, (d) select the transformed callus that expresses the exogenous gene , (e) cultivate the selected callus in suspension culture in differentiation medium that does not contain plant hormone for less than 20 days to induce embryoid formation, (f) germinates r embryoids in a medium that has a source of nitrogen selected from the group consisting of asparagine, glutamine or asparagine as well as glutamine and that does not contain plant hormone, to produce young transgenic cotton plants, and (g) grow cotton plants Young transgenic in a medium that has 10 g / l glucose and 10 g / l sucrose as a carbon source and does not contain plant hormone, to produce transgenic cotton plants, in which the culture media used in the stages (b ) - (f) have glucose as the only carbon source and in which step (e) is performed at a pH between 5.8 and 7.5. |
| priorityDate | 1999-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 66.