| abstract |
A method of producing L-glutamic acid, which comprises the step of culturing a coryneform bacterium that expresses an enzyme encoded by a gene that biosynthesizes L-glutamic acid, selected from the group consisting of glutamate dehydrogenase (GDH), citrate synthase ( CS), isocitrate dehydrogenase (ICDH), pyruvate dehydrogenase (PDH) and aconitase (ACO), and in which a promoter sequence of said gene that biosynthesizes L-glutamic acid, located on a chromosome of the bacterium, contains at least a sequence selected from the group consisting of i) a TTGTCA, TTGACA or TTGCCA sequence in the -35 region of the promoter sequence; ii) the TATAAT sequence in the -10 region of the promoter sequence; and iii) a combination of (i) and (ii). |