Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f7ddc4774cf5f8a0bda44a780d73c53d |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-241 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
filingDate |
2004-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2010-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_15f1242ae7146b6badc9bc6c9008134d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ceba070752048340ff9a5b6d9aadd3d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_73a8d4cc16357235942e34e3fda3ffb9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_131840b3461ede53c01e67e73532f90f |
publicationDate |
2010-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
ES-2331573-T3 |
titleOfInvention |
EXPRESSION VECTOR FOR THE SECRETION OF AN ANTIBODY FRAGMENT USING A SIGNAL SEQUENCE OF E. COLI AND PROCEDURE FOR SERIAL PRODUCTION OF THE ANTIBODY FRAGMENT. |
abstract |
A method of producing an antibody fragment, comprising the steps of: a) preparing an expression vector comprising a gene encoding a light chain of the antibody fragment fused with the E. coli thermostable enterotoxin signal sequence and a gene encoding a heavy chain of the antibody fragment fused with the signal sequence of protein A of the outer membrane of E. coli, in which the expression of the genes encoding the light chain and heavy chain is regulated by a single promoter; b) transform a microorganism with the expression vector; c) cultivate the transformed microorganism in a medium; and d) collecting the antibody fragment secreted from the transformed microorganism within the medium or periplasmic space of the microorganism. |
priorityDate |
2003-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |