| abstract |
Method for producing a biologically active neublastin polypeptide, which comprises culturing a cell comprising an expression vector comprising a nucleic acid comprising a promoter sequence operably linked to a nucleotide sequence encoding a signal peptide and a neublastin polypeptide, wherein said nucleotide sequence does not encode a pro-region of neublastin, and wherein said signal peptide is an immunoglobulin signal peptide, a TGF-beta signal peptide, a GDF signal peptide, an IGF signal peptide , a BMP signal peptide, a neurotrophin signal peptide, a PDGF signal peptide, an EGF signal peptide, an insulin signal peptide, an ADH signal peptide, an LH signal peptide, an FSH signal peptide, a ACTH signal peptide, an MSH signal peptide, a TSH signal peptide, a DHEA signal peptide, an interleukin signal peptide, a neur signal peptide turbine, a GDNF signal peptide, a persephin signal peptide, an NGF signal peptide, a signal peptide that is the signal peptide contained in SEQ ID NO: 70, or a native neublastin signal peptide. |