http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2321492-T3
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bcb146c68b67089013b4c7a614504254 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-255 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-25375 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1017 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-08 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N- http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 |
filingDate | 2002-09-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2009-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_92d2a1bea48dae4999dabff1247411c6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa10544979860fb55f2f11b40943cf57 |
publicationDate | 2009-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | ES-2321492-T3 |
titleOfInvention | INSULATION AND PURIFICATION OF NUCLEIC ACIDS. |
abstract | A method for separating nucleic acids from materials that are not nucleic acids in a nucleic acid solution, comprising: a) combining cellulose with a solution containing nucleic acids and materials that are not nucleic acids to produce a first combination; b) adjust the concentration of polyethylene glycol at a concentration between 5% and 15%, and the concentration of salts at a concentration between 0.25 M and 5.0 M, thereby producing a second combination comprising bound nucleic acids to cellulose, and in which nucleic acids bind to cellulose when nucleic acids are in a state not aggregated in the solution; c) separating the cellulose-bound nucleic acids from the second combination; d) contacting the cellulose bound nucleic acids separated in c) with an elution buffer to release the bound nucleic acids from the cellulose and separating them into the elution buffer; and e) separating the cellulose from the elution buffer to provide nucleic acids that are substantially free of non-nucleic acid materials, in which the separations in step c) and e) are performed using a centrifuge, a column or manually . |
priorityDate | 2001-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.