| abstract |
A recombinant luciferase that has luciferase activity and an amino acid sequence that differs from the wild-type luciferase of Photinus pyralis, Luciola mingrelica, Luciola cruciata, Luciola lateral, Hotaria paroula, Pyrophorus plagiophthalamus, Lampyris noctiluca, Pyrocoelinus nayakovanica nayakovanica nayakovanica nayakovanica nayakovanica nayakovanica nayakovanica nayakovanica nayakovanica nayakovanica nayakovanica nayakovanica nayakovanica nayakovanica inayak the sequence of the recombinant luciferase, the amino acid residue corresponding to threonine 214 in wild-type luciferase of Photinus pyralis or glycine 216 of wild-type luciferase of Luciola mingrelica, or asparagine 216 in wild-type luciferases of Luciola cruciata or Luciola lateralis is mutated compared to the corresponding amino acid that appears in the corresponding wild-type luciferase sequence, such that the recombinant luciferase has increased thermostability compared to the corresponding wild-type luciferase. |