http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2301271-A1

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assignee http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7da5c8e8c3e8261efe7e698c7198681b
classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34
classificationIPCAdditional http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858
filingDate 2004-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_905296eac7d72e87f95ea1e90796ca10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ad93529e75fed351450e92808ed8eba
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_90669795a652d3a89c10b86d1d738db9
publicationDate 2008-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber ES-2301271-A1
titleOfInvention PROCEDURE FOR AMPLIFICATION OF NUCLEIC ACIDS SEQUENCES NOT EXPANDABLE BY PCR.
abstract Sequence amplification procedurennucleic acids not amplifiable by PCR.n n n The object of the present invention is annucleic acid sequence amplification procedurenwhich cannot be amplified by standard PCR, which comprisesnTwo stages: a) nonspecific pre-amplification, withnrandom primers and the enzyme polymerase \ Phi29, using MDAn(Multiple displacement amplification) and b) amplification withnspecific primers, by PCR (Polymerase chain reaction).nSaid procedure allows amplification, and therefore, thendetection, analysis and study of specific acid sequencesnnucleic acids (DNA or RNA) in those cases in which the reactionnPCR standard is unable to produce positive resultsndue to the low number of copies of the specific sequence thatnwant to amplify or detect, or due to the presence of substancesnPCR inhibitors at concentrations higher than allowednby standard PCR.
priorityDate 2004-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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