http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2301271-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7da5c8e8c3e8261efe7e698c7198681b |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 |
filingDate | 2004-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_905296eac7d72e87f95ea1e90796ca10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ad93529e75fed351450e92808ed8eba http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_90669795a652d3a89c10b86d1d738db9 |
publicationDate | 2008-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | ES-2301271-A1 |
titleOfInvention | PROCEDURE FOR AMPLIFICATION OF NUCLEIC ACIDS SEQUENCES NOT EXPANDABLE BY PCR. |
abstract | Sequence amplification procedurennucleic acids not amplifiable by PCR.n n n The object of the present invention is annucleic acid sequence amplification procedurenwhich cannot be amplified by standard PCR, which comprisesnTwo stages: a) nonspecific pre-amplification, withnrandom primers and the enzyme polymerase \ Phi29, using MDAn(Multiple displacement amplification) and b) amplification withnspecific primers, by PCR (Polymerase chain reaction).nSaid procedure allows amplification, and therefore, thendetection, analysis and study of specific acid sequencesnnucleic acids (DNA or RNA) in those cases in which the reactionnPCR standard is unable to produce positive resultsndue to the low number of copies of the specific sequence thatnwant to amplify or detect, or due to the presence of substancesnPCR inhibitors at concentrations higher than allowednby standard PCR. |
priorityDate | 2004-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 68.