http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2290613-T3

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publicationDate 2008-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber ES-2290613-T3
titleOfInvention COMPOSITIONS THAT INCLUDE VIRUSES AND METHODS TO CONCENTRATE VIRUS PREPARATIONS.
abstract A method of purifying a virus preparation comprising: (a) subjecting the virus preparation to anion exchange chromatography, wherein the virus is eluted as a product of a virus preparation from an anion exchange chromatographic medium; (b) adding a polyhydroxylated hydrocarbon to the virus product preparation of step (a) so that the concentration of polyhydroxylated hydrocarbon in the preparation reaches a final concentration of about 25% or more; (c) increasing the virus concentration in the virus preparation product of step (b) by applying pressure to the preparation such that diluent is separated from the virus preparation through a filter while the virus is retained ; and (d) subjecting the product of the concentrated virus preparation of step (c) to one or more stages of additional treatments.
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