http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2281354-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c32775641a2462e0c791af0b9b077e08 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6839 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566 |
| filingDate | 2000-08-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2007-10-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8f57344020b2033feca1bb70b42916bf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0cc8016592b7be556ffb1ea9b45485ea http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eac3a6b950c4ae78243941e1f83bab87 |
| publicationDate | 2007-10-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2281354-T3 |
| titleOfInvention | DETECTION OF DIFFERENCES IN NUCLEIC ACIDS THROUGH THE INHIBITION OF THE SPONTANEOUS MIGRATION OF DNA RAMIFICATION. |
| abstract | A method for detecting the presence of a difference between a target nucleic acid sequence and a reference nucleic acid sequence, comprising: (a) amplification of the target nucleic acid sequence by polymerase chain reaction, using a P2 primer, a P4 primer with a 3 '' region capable of hybridizing to the target sequence and a T region in the 5 '' tail that is not complementary to the target sequence, and a P5 primer that is capable of hybridizing to the target sequence at a 3 '' location of the sequence capable of hybridizing to said 3 '' fraction of primer P4; in which either: primer P2 is a mixture of primer P2 with a first marker and primer P2 with a second marker, or primer P4 has a first marker and primer P5 has a second marker; (b) the formation of a target partial duplex with tail of the target sequence, said partial duplex having one of said first marker or said second marker, and a tail of two non-complementary regions in which a first region is the P5 sequence or its complement and the second region is the sequence T or its complement; (c) amplification of the reference sequence by polymerase chain reaction, using a P2 primer, a P4 primer with a 3 '' region capable of hybridizing to the reference sequence and a T region in the 5 'tail 'which is not complementary to the reference sequence, and a P5 primer that is capable of hybridizing to the reference sequence substantially adjacent, in the 3' 'direction, to the sequence capable of hybridizing to said 3' 'fraction of primer P4. |
| priorityDate | 1999-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 267.