http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2220180-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1576fbe001d61ee8930a02549a9d5679 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7076dedc2d2a657bb9fccb6f5dbf0296 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6865 |
| filingDate | 2002-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2005-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a93f9c0098ce823f5b43ddb4b7907dde http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f44ea8b5da6b534723ea505dc55e0779 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_43a4a07feac4869bc0a5d24b5d85bdcc |
| publicationDate | 2005-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2220180-B1 |
| titleOfInvention | BRUCELLA SPP SPECIFIC DNA SEQUENCES DETECTION PROCEDURE THROUGH POLYMERASE CHAIN (PCR) REACTION COUPLED TO AN ENZYMOINMUNOENSAY (ELISA). |
| abstract | Method for detecting Brucella spp- specific DNA sequences by polymerase chain reaction (PCR) coupled to an enzyme-linked immunoassay (ELISA).n n n Method for detecting specific DNA sequences from Brucella spp. by polymerase chain reaction (PCR) coupled to an enzyme immunoassay (ELISA). After selective amplification of a Brucella 223 bp sequence using oligonucleotides B4 and B5, the resulting amplified products labeled with digoxigenin (DIG) are hybridized with a biotinylated capture probe. Subsequently, these hybrids are captured on streptavidin coated plates and detected using an anti-DIG Fab / peroxidase conjugate. The proposed technique has been much more sensitive than conventional PCR and bacteriological methods and more specific than the usual serological methods, allowing its use for the implementation of an easy and rapid molecular diagnostic procedure for Brucella spp infection . in human blood samples and in other clinical samples, the monitoring of the response to treatment and the early detection of recurrences avoiding the risk of germ manipulation. |
| priorityDate | 2002-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 43.