| abstract |
Method for preparing a DNA comprising sequences derived from the genomic RNA (gRNA) of a coronavirus, said sequences having a homology of at least 60% with respect to the natural sequence of the virus and coding for an RNA-dependent RNA polymerase and therefore less a structural or non-structural protein, in which the fragment of said DNA can be transcribed into RNA and assembled to a virion, said method comprising the steps of cloning a defective coronavirus interfering genome under the expression of a promoter on a chromosome Artificial bacterial (BAC) and insertion back into the defective genome sequences deleted in said genome. |