| abstract |
Development of an artificial skin by culturing keratinocytes on a fibrin base and human fibroblasts and method of preparing this skin for transplantation. Artificial skin obtained by cell culture of keratinocytes on a base consisting of a human fibrin gel populated with human fibroblasts. This base allows very rapid development of keratinocytes, detecting proteins from the dermal-epidermal junction in this artificial skin that facilitate subsequent transplant success. This artificial skin requires, to be used in the clinic, a previous preparation that consists of fixing it to a solid support and a system that enables transport to the transplant site without breaking it. The artificial skin described here can be used for the treatment of major burns, chronic skin ulcers or used, through the use of genetically modified cells, as a basis for gene therapy. |