http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2121561-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1b74695f4c6259a8598b722c1ef6eefa |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06 |
| filingDate | 1997-05-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b243392b1d3c8103e51ae25ec6f2bd08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dcafd0aedbf752db9fc14c6ed159c5b5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b0fee93c75e82ea0182c855b2845e85b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c91c9ddcb8b4d743c6d66d2adffe6b0a |
| publicationDate | 1998-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2121561-A1 |
| titleOfInvention | NITRATE REDUCTASE THERMOSTABLE APPLICABLE FOR THE DETECTION OF NITRATES AT HIGH TEMPERATURE. |
| abstract | THERMOSTABLE NITRATE REDUCTASE APPLICABLE FOR THE DETECTION OF NITRATES AT HIGH TEMPERATURES, WHICH CONSISTS OF CLONING THE REGION OF DNA THAT CODES THE THERMOSTABLE NITRATE REDUCTASE APPLICABLE FOR THE DETECTION OF NITRATES AT HIGH TEMPERATURES, FROM A DNA LIBRARY OF THE BACTERIA THERMUS THERMOPHILUS HB8 , USING FOR IT A SPECIFIC DNA PROBE FOR ITS DETECTION. A FRAGMENT OF 8.2 KILO-PAIRS OF BASES, INCLUDED BETWEEN TWO PSTI RESTRICTION TARGETS, WAS ISOLATED FROM THIS REGION AND CLONED INTO THE BIFUNCTIONAL VECTOR PMK18. THE PMK18 VECTOR IS CHARACTERIZED BY HAVING TWO FUNCTIONAL ORIGINS OF REPLICATION FROM ESCHERICHIA COLI AND THERMUS THERMOPHILUS, A GENE THAT CONFERS THERMOSTABLE RESISTANCE TO KANAMYCIN PROTECTED BY PATENT OF INVENTION 9301522 APPLIED FOR IN SPAIN, AN INDUCIBLE PROMOTER IN ESCHERICHIA COLI AND A CLONING REGION TWO MULTIPLE RESTRICTION TARGETS. WHEN THIS CONSTRUCTION IS INTRODUCED IN THERMUS THERMOPHILUS HB27 OR IN ESCHERICHIA COLI, A SOLUBLE ENZYME OF 192 KILODALTONS OF MOLECULAR SIZE IS SYNTHESIZED, CAPABLE OF CATALYSING THE REDUCTION OF NITRATE OR NITRITE AT AN OPTIMUM TEMPERATURE OF 80 C. |
| priorityDate | 1997-05-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 26.