http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2075802-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a58aab8ab8ea1b2493e25c88e5bd7338 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-1228 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56911 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 |
| filingDate | 1993-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1996-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_871439b1e0cf0ef1f1f60b76853e6dd1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e74b6f5f2c104526d7f3b669665abffd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ad5aa15119b452bf0e49c95f0aedc6e2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f80f42839af62ceb1bfd76c3b52db53d |
| publicationDate | 1996-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2075802-B1 |
| titleOfInvention | PROCEDURE FOR THE SENSITIVE AND SPECIFIC DETECTION OF ERWINIA CAROTOVORA SUBSP. ATROSEPTICA THROUGH "ELISA-ENRICHMENT". |
| abstract | PROCEDURE FOR THE SENSITIVE AND SPECIFIC DETECTION OF ERWINIA CAROTOVORA SUBSP. ATROSEPTICA THROUGH "ELISA-ENRICHMENT". THE PROCEDURE PART OF THE INSOLUBILIZATION OF ANTIGENS OF THE PATOGEN AGENT ERWINIA CAROTOVORA SUBSP. ATROSEPTICA (ECA) IN MICROPLATES, THROUGH MONOCLONAL OR POLYCLONE ANTIBODIES. LATER, IN THE SAME MICROPLATE, THE ADDED SAMPLE OF STUDY IS ADDED WITH A SELECTIVE MEDIA FOR ERWINIAS (D-PEM) AND INCUBATES IN ANAEROBIOSIS AT 25 C FOR 24-48 HOURS. REACTION IS DISCLOSED THROUGH BIOTINILATED ECA-SPECIFIC MONOCLONAL ANTIBODIES (4G4) AND SUBSEQUENT ADDITION OF STREPTOAVIDINE MARKED WITH AN ENZYME OR DIRECTLY WITH 4G4 ANTI-POSITIVE APPLICATORS AND A DASPURA DIGITAL ORIGIN (ELISA-DAS OR DASUA DASA) ADDITION OF AN ANTI-SPECIES CONJUGATE (ELISA-DASI). HYDROLYSIS OF THE ENZYMATIC SUBSTRATE IMPLIES THE APPEARANCE OF A COLORED PRODUCT THAT IS PHOTOCOLORIMETRICALLY ASSESSED. THE PARALLEL PERFORMANCE OF THE SAMPLE IN MICROPLATES ENRICHED WITH D-PEN OR WITHOUT ENRICHMENT, ALLOWS TO CONCLUDE ON THE PRESENCE AND AMOUNT OF LIVE CELLS OF ECA CAPABLE OF CAUSING DISEASE IN TUBERCULOS OR OTHER VEGETABLE MATERIAL. |
| priorityDate | 1993-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 21.