| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ef5c40410de40f6f43f3351aad1280d7 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P3-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P3-08 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-28
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P3-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P3-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-575
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-17
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate |
1988-08-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4eedf926a13abc552917e3030dfb39ac
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_26c73ade226b4f509c7e06d6c54539a0 |
| publicationDate |
1989-11-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
ES-2010299-A6 |
| titleOfInvention |
A PROCEDURE FOR THE PREPARATION OF HUMAN INSULIN ANALOGS PRESENTING BIOLOGICAL ACTIVITY. |
| abstract |
A PROCEDURE FOR THE PREPARATION OF HUMAN INSULIN ANALOGS PRESENTING BIOLOGICAL ACTIVITY. INCLUDES: INSERTING THE SEQUENCE OF THE INSULIN PRECURSOR ISOLATED FROM THE EXPRESSION PLASMIDE IN A VECTOR OF THE MONOFILAMENTARY CIRCULAR BACTERIOPHAGUS M13; MERGING THE MONOFILAMENT GENOME A CYCLAD COMPLEMENTARY DNA STRAIN CONTAINING THE DESIRED SEQUENCE SURROUNDED BY COMPLETELY HOMOLOGATED SEQUENCES TO INSULIN SEQUENCES IN CIRCULAR DNA; PROLONGING THE PRIMER IN VITRO OVER THE COMPLETE LENGTH OF THE CIRCULAR GENOME BIOCHEMICALLY USING KLENOW POLYMERASE, OBTAINING MONOFILAMENTAL FAGUSES WHEN GROWING IN E.COLI ALLOWS TO INSULATE DOUBLE-STRED DNA WITH THE SEQUENCE ISOLATE A RESTRICTING FRAGMENT OF THIS DNA AND REINSTART IT IN THE EXPRESSION VECTOR; TRANSFORM IT INTO A GUEST, CULTIVATE AND ISOLATE THE INSULIN PRECURSOR FROM THE CULTIVATION MEDIA. THESE PRODUCTS HAVE USEFULNESS IN THE TREATMENT OF DIABETES MELLITUS. |
| priorityDate |
1988-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |