| abstract |
Provided herein are methods and compositions for synthesizing 5'Capped RNAs wherein the initiating capped oligonucleotide primers have the general form <sup>m7</sup>Gppp[N<sub>2'Ome</sub>]<sub>n</sub>[N]<sub>m</sub> wherein <sup>m7</sup>G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, "n" can be any integer from 0 to 4 and "m" can be an integer from 1 to 9. |