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filingDate 2021-02-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2022-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-4103740-A1
titleOfInvention Nanopore device and methods of detecting and classifying charged particles using same
abstract A method of determining an oligonucleotide methylation percentage includes providing a 3D nanopore device having top and bottom chambers, and a 3D nanochannel array disposed therein. The method also includes purifying an oligonucleotide, and functionalizing the 3D nanochannel array by coupling an oligonucleotide probe. The method further includes forming an oligonucleotide solution having a known concentration, and adding the oligonucleotide solution to the top and bottom chambers. Moreover, the method includes placing top and bottom electrodes in the top and bottom chambers respectively, applying an electrophoretic bias between the top and bottom electrodes, applying a selection bias across first and second gating nanoelectrodes, applying a sensing bias through a sensing nanoelectrode in the 3D nanopore device. In addition, the method includes detecting an output current from the sensing nanoelectrode, and analyzing the output current from the sensing nanoelectrode to determine a methylation percentage of the oligonucleotide.
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