http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-4100716-A1

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filingDate 2021-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_da9ec20a24b204b6395bdc363beb62c7
publicationDate 2022-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-4100716-A1
titleOfInvention Particle analysis method and particle analyzer
abstract Provided is a means capable of obtaining more clinically useful information when particles contained in a blood sample are analyzed using a metachromatic orthochromatic dye. In a particle analysis method of analyzing particles contained in a blood sample, the particles are stained with a metachromatic orthochromatic dye, the stained particles are irradiated with light, intensity of a first fluorescence derived from a stacking component of the metachromatic orthochromatic dye and intensity of a second fluorescence derived from an intercalation component of the metachromatic orthochromatic dye are measured, the first fluorescence and the second fluorescence being emitted by each particle contained in the blood sample, the intensity of the first fluorescence and the intensity of the second fluorescence emitted by each of the particles are normalized by the size of each of the particles to obtain a fluorescence concentration of each of the first fluorescence and the second fluorescence in each of the particles, each of the particles is clustered into a plurality of particle clusters including at least two of an erythrocyte cluster, a platelet cluster and a nucleated cell cluster, in a two-dimensional plot of the fluorescence concentration obtained by the normalization, and a size histogram in which the size of each particle included in the particle cluster is a class is created for at least one particle cluster included in the plurality of particle clusters.
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