http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-4089110-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_48c86a2b5a74a8b0b72f86a2ec021389 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4d5d546aa6cbfb52c137af7cafc36bf4 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-41 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-065 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-005 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 |
| filingDate | 2020-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_242b05a9c1366dec91d6d0c62c59e81f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ee583617fcc10e0ce9c9b26885c7a460 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9cb6906f6431170dfd891d871e3d82cb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cb21846aa980edeea6f3c1b0a777a2bb |
| publicationDate | 2022-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-4089110-A1 |
| titleOfInvention | Antibody population uniformly including antibodies having left-right asymmetric sugar chain, and method for producing same |
| abstract | Provided are a method for preparing a population of antibodies comprising homogeneous antibodies each having left-right asymmetric sugar chains, and a population of homogeneous antibodies each having left-right asymmetric sugar chains, obtained by the method. The present invention provides a method for producing a population of antibodies comprising homogeneous antibodies in which N-linked complex sugar chains attached to asparagine (Asn) at position 297 of CH domains of Fc regions of two heavy chains on the left and the right of each antibody are sugar chains structurally different from each other, the method comprising the steps of: (i) cleaving sugar chains attached to two heavy chains on the left and the right of each antibody in an antibody composition with endo-β-N-acetylglucosaminidase (ENGase), and purifying and isolating antibodies from which the sugar chains of both the heavy chains have been cleaved; (ii) mixing a population of the antibodies from which the sugar chains of both the heavy chains have been cleaved, obtained in the step (i), any sugar chain X in an oxazoline form or an oxazoline-generating sugar derivative, the sugar chain of which is sugar chain X, and ENGase modified so as to suppress sugar chain cleavage activity and improve sugar chain transfer activity, to prepare antibodies in which the sugar chain X is attached to only one of the two heavy chains on the left and the right, followed by purification and isolation of the antibodies; and (iii) mixing the antibodies in which the sugar chain X is attached to only one of the two heavy chains on the left and the right, obtained in the step (ii), sugar chain Y in an oxazoline form which is structurally different from the sugar chain X, or an oxazoline-generating sugar derivative, the sugar chain of which is sugar chain Y, and ENGase modified so as to suppress sugar chain cleavage activity and improve sugar chain transfer activity, to prepare antibodies in which the sugar chain Y is attached to the other heavy chain of the two heavy chains on the left and the right and in which the sugar chains attached to the two heavy chains on the left and the right are sugar chains structurally different from each other, followed by purification and isolation of the antibodies. |
| priorityDate | 2020-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 63.